unassigned: Apoptosis is responsible for eliminating damaged and virus-infected cells, regulating normal cell turnover, and maintaining the immune system's development and function. Caspases play a vital role in both mammal and invertebrate apoptosis. is a generalist insect herbivore that is one of the most destructive pests in tropical and subtropical areas and attacks a wide range of commercially important crops. Although is a model organism in the study of baculovirus infection, its apoptotic pathway has not been explored.
unassigned: We cloned a new caspase gene named in using Rapid Amplification of cDNA Ends (RACE). We then measured caspase activity on synthetic caspase substrates and ' effector caspas... More
unassigned: Apoptosis is responsible for eliminating damaged and virus-infected cells, regulating normal cell turnover, and maintaining the immune system's development and function. Caspases play a vital role in both mammal and invertebrate apoptosis. is a generalist insect herbivore that is one of the most destructive pests in tropical and subtropical areas and attacks a wide range of commercially important crops. Although is a model organism in the study of baculovirus infection, its apoptotic pathway has not been explored.
unassigned: We cloned a new caspase gene named in using Rapid Amplification of cDNA Ends (RACE). We then measured caspase activity on synthetic caspase substrates and ' effector caspase. SlDronc's function in the apoptotic pathway and its interaction with caspase inhibitors were also tested in SL2 cells.
unassigned: We found that the initiator caspase SlDronc cleaved and activated effector caspase in . SlDronc overexpression induced apoptosis in SL2 cells, and knockdown decreased apoptosis induced by UV irradiation in SL2 cells. Our results indicate that SlDronc acts as an apoptotic initiator caspase in . Additionally, we found that processed forms of SlDronc increased in the presence of N-terminally truncated inhibitors of apoptosis (SlIAP) and that SlDronc was inhibited by P49. This study contributes to the further understanding of apoptotic pathway and may facilitate future studies on baculovirus infection-induced apoptosis.
