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Proteins, Expression, Isolation and Analysis | … min, samples were sonicated and centrifuged at 10,000 × g for 20 min. The resulting supernatant was purified with Glutathione Resin (GenScript, China). Protein concentrations were determined using a Bio-Rad Protein Assay Kit I. For immunoblotting, the proteins were … | Get A Quote |
Reactive oxygen species (ROS) are plant metabolic and signaling molecules involved in responses to various external stresses, but the existence of ROS receptors and how plants respond to ROS remain largely unknown. Here we report that the plasma membrane-localized phospholipase D δ (PLDδ) protein is crucial for sensing heat shock-induced ROS to initiate reorganization of guard cell microtubules in Arabidopsis cotyledons. Heat shock of wild-type Arabidopsis cotyledons stimulated ROS production which disrupted microtubule organization and induced stomatal closure, whereas this process was markedly impaired in pldδ mutants. Moreover, the wild-type PLDδ, but not the Arg-622-mutated PLDδ, complemented the pldδ... More