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Proteins, Expression, Isolation and Analysis | … LTAs were isolated from L. plantarum K8 (KCTC 10887BP; pLTA) and S. aureus (ATCC 25923; aLTA) as previously described [8]. Protein and endotoxin contamination were examined using silver staining and an endotoxin assay kit (GenScript, NJ, USA), respectively, and it is … | Get A Quote |
Lipoteichoic acid (LTA), a cell wall component of gram-positive bacteria, up-regulates inflammatory cytokine production through the toll-like receptor 2 (TLR2) signaling pathway, and also contributes to anti-inflammatory responses against immune cells stimulated by lipopolysaccharides. In the current study, we examined the effects of LTAs isolated from Staphylococcus aureus (aLTA) and Lactobacillus plantarum (pLTA) on the aggravation and alleviation of atopic dermatitis (AD). aLTA strongly induced CCL2 production in THP-1 cells. CCL2 was regulated by the TLR2 pathway including the activation of IRAK2, NF-κB and JNK. CCL2 induced Th2 polarization of CD4+T cells through induction of interleukin (IL)-2, -4, and ... More