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Homogeneous batch micro-crystallization of proteins from ammonium sulfate

Acta Crystallogr D Struct Biol. 2021-01; 
Claudia Stohrer, Sam Horrell, Susanne Meier, Marta Sans, David von Stetten, Michael Hough, Adrian Goldman, Diana C F Monteiro, Arwen R Pearson
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Codon Optimization AcNiR protein was prepared following a previously published protocol (Horrell et al., 2016). In brief, a pET-26b plasmid containing the codon- optimized gene for AcNiR (GenScript) was transformed into E. coli BL21 (DE3) cells Get A Quote

摘要

The emergence of X-ray free-electron lasers has led to the development of serial macromolecular crystallography techniques, making it possible to study smaller and more challenging crystal systems and to perform time-resolved studies on fast time scales. For most of these studies the desired crystal size is limited to a?few micrometres, and the generation of large amounts of nanocrystals or microcrystals of defined size has become a bottleneck for the wider implementation of these techniques. Despite this, methods to reliably generate microcrystals and fine-tune their size have been poorly explored. Working with three di

关键词

ammonium sulfate, batch crystallization, microcrystals, serial crystallography