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Proteins, Expression, Isolation and Analysis | … The cell lysate supernatant was loaded onto a column packed with the anti-Flag G1 affinity resin (GenScript), washed with lysis buffer, and finally eluted with the 0.2 mg/mL flag peptide (GenScript). The TEV enzyme was added to digest the flag-tag overnight at 4 C. The … | Get A Quote |
Post-translational modification (PTM) on protein plays important roles in the regulation of cellular function and disease pathogenesis. The systematic analysis of PTM dynamics presents great opportunities to enlarge the target space by PTM allosteric regulation. Here, we presented a framework by integrating the sequence, structural topology, and particular dynamics features to characterize the functional context and druggabilities of PTMs in the well-known kinase family. The machine learning models with these biophysical features could successfully predict PTMs. On the other hand, PTMs were identified to be significantly enriched in the reported allosteric pockets and the allosteric potential of PTM pockets wer... More