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Proteins, Expression, Isolation and Analysis | … Equal amounts of GST‐PYL4 and His‐StLTP10 sonicated lysates were mixed with high‐affinity GST resin (GenScript) and incubated for 2 hr at 4C with constant rotation. The bound proteins were then eluted with fresh glutathione elution buffer. Afterwards, the proteins were … | Get A Quote |
Nonspecific lipidtransfer proteins (nsLTPs), which are small, cysteine-rich proteins, belong to the pathogenesis-related protein family, and several of them act as positive regulators during plant disease resistance. However, the underlying molecular mechanisms of these proteins in plant immune responses are unclear. In this study, a typical nsLTP gene, StLTP10, was identified and functionally analysed in potato. StLTP10 expression was significantly induced by Phytophthora infestans, which causes late blight in potato, and defence-related phytohormones, including abscisic acid (ABA), salicylic acid, and jasmonic acid. Characterization of StLTP10-overexpressing and knockdown lines indicated that StLTP10 positive... More