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Osteopontin Blockade Immunotherapy Increases Cytotoxic T Lymphocyte Lytic Activity and Suppresses Colon Tumor Progression

Cancers (Basel). 2021-02; 
John D Klement, Dakota B Poschel, Chunwan Lu, Alyssa D Merting, Dafeng Yang, Priscilla S Redd, Kebin Liu
Products/Services Used Details Operation
Gene Synthesis … To create the OPN Ko cell line, HEK293FT cells were co-transfected with pCMV-VSV-G (#8454, Addgene, Watertown, MA, USA), psPAX2 (#12260, Addgene, Watertown, MA, USA) and lentiCRISPRv2 (Genscript, Piscataway, NJ, USA) plasmids using Lipofectamine 2000 (Life … Get A Quote

摘要

Human colorectal cancers are mostly microsatellite-stable with no response to anti-PD-1 blockade immunotherapy, necessitating the development of a new immunotherapy. Osteopontin (OPN) is elevated in human colorectal cancer and may function as an immune checkpoint. We aimed at elucidating the mechanism of action of OPN and determining the efficacy of OPN blockade immunotherapy in suppression of colon cancer. We report here that OPN is primarily expressed in tumor cells, myeloid cells, and innate lymphoid cells in human colorectal carcinoma. Spp1 knock out mice exhibit a high incidence and fast growth rate of carcinogen-induced tumors. Knocking out Spp1 in colon tumor cells increased tumor-specific CTL cytotoxici... More

关键词

MSS, OPN neutralization, PD-L1, cytotoxic T lymphocytes, immune checkpoint, osteopontin