| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | … The region of the human AS3MT gene from nucleotide positions at -833 to +630 (where the nucleotide is immediately upstream of the putative transcription starting site is denoted as “-1”) was chemically synthesized by GenScript (Piscataway, NJ, USA) … | Get A Quote |
The human enzyme As(III) S-adenosylmethionine methyltransferase (AS3MT) catalyzes arsenic biotransformations and is considered to contribute to arsenic-related diseases. AS3MT is expressed in various tissues and cell types including liver, brain, adrenal gland, and peripheral blood mononuclear cells but not in human keratinocytes, urothelial, or brain microvascular endothelial cells. This indicates that AS3MT expression is regulated in a tissue/cell type-specific manner, but the mechanism of transcriptional regulation of expression of the AS3MT gene is not known. In this study, we define the DNA sequence of the core promoter region of the human AS3MT gene. We identify a GC box in the promoter to which the stres... More
