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Soluble Expression and Catalytic Properties of Codon-Optimized Recombinant Bromelain from MD2 Pineapple in Escherichia coli

Protein J. 2021-03; 
Rafida Razali, Cahyo Budiman, Khairul Azfar Kamaruzaman, Vijay Kumar Subbiah
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Gene Synthesis … The DNA sequence of fruit bromelain MD2Bro was retrieved from the MD2 pineapple genome listed under the GenBank accession number OAY85858.1. Codon optimization was then performed with OptimumGene™ software of GenScript (NJ, USA), and subsequently, the … Get A Quote

摘要

Bromelain, a member of cysteine proteases, is found abundantly in pineapple (Ananas comosus), and it has a myriad of versatile applications. However, attempts to produce recombinant bromelain for commercialization purposes are challenging due to its expressibility and solubility. This study aims to express recombinant fruit bromelain from MD2 pineapple (MD2Bro; accession no: OAY85858.1) in soluble and active forms using Escherichia coli host cell. The gene encoding MD2Bro was codon-optimized, synthesized, and subsequently ligated into pET-32b( +) for further transformation into Escherichia coli BL21-CodonPlus(DE3). Under this strategy, the expressed MD2Bro was in a fusion form with thioredoxin (Trx) tag a... More

关键词

Ananas comosus, Bromelain, Codon optimization, Cysteine proteases, MD2 pineapple