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Culture media optimization for Chinese hamster ovary cell growth and expression of recombinant varicella-zoster virus glycoprotein E

Cytotechnology. 2021-04; 
Kwang Sung Kim, Shin Ae Park, Seo Ri Wui, Ara Ko, Na Gyong Lee
Products/Services Used Details Operation
Gene Synthesis … 1a) and cloned into the EcoRI and XbaI sites of pGN-M vector containing the CMV promoter and the dihydrofolate reductase gene (GenScript, NJ, USA), yielding the expression plasmid pGN-M_VZV gE as shown in Fig. 1b. CHO DG44 cells (Urlaub et al … Get A Quote

摘要

关键词

Chinese hamster ovary (CHO) cell clone, Culture media optimization, Fed-batch culture, Immunogenicity, Vaccine antigen, Varicella-zoster virus glycoprotein E