| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | Plasmids encoding the I53-50A1 trimer and I53-50B.4PT1 pentamer fused with a C-terminal octahistidine tag (HHHHHHHH) were codon optimized for expression in Escherichia coli cells,synthesized by GenScript, and introduced into the pET28b prokaryotic expression vector using the NcoI and HindIII restriction enzyme sites. Before formulation in MF59-like adjuvant, residual endotoxin of HexaPro-based immunogens was again tesed using by ToxinSensorTM Chromogenic LAL Endotoxin Assay Kit (Genscript, Cat#L00350). | Get A Quote |
| Endotoxin Detection & Removal System | Get A Quote |
Emerging SARS-CoV-2 variants of concern (VOCs) harboring multiple mutations in the spike protein raise concerns on effectiveness of current vaccines that rely on the ancestral spike protein. Here, we design a quadrivalent mosaic nanoparticle vaccine displaying spike proteins from the SARS-CoV-2 prototype and 3 different VOCs. The mosaic nanoparticle elicits equivalent or superior neutralizing antibodies against variant strains in mice and non-human primates with only small reduction in neutralization titers against the ancestral strain. Notably, it provides protection against infection with prototype and B.1.351 strains in mice. These results provide a proof of principle for the development of multivalent vacci... More
