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Dual-polarity voltage imaging of the concurrent dynamics of multiple neuron types

Science. 2022-11; 
Madhuvanthi Kannan, Ganesh Vasan, Simon Haziza, Cheng Huang, Radosław Chrapkiewicz, Junjie Luo, Jessica A Cardin, Mark J Schnitzer, Vincent A Pieribone
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Gene Synthesis … the four improved FRET-opsin GEVIs, we synthesized codon-optimized transcripts for Ace-mNeon2, VARNAM2, pAce, and pAceR using a commercial gene synthesis service (GenScript Get A Quote

摘要

Genetically encoded fluorescent voltage indicators are ideally suited to reveal the millisecond-scale interactions among and between targeted cell populations. However, current indicators lack the requisite sensitivity for in vivo multipopulation imaging. We describe next-generation green and red voltage sensors, Ace-mNeon2 and VARNAM2, and their reverse response-polarity variants pAce and pAceR. Our indicators enable 0.4- to 1-kilohertz voltage recordings from >50 spiking neurons per field of view in awake mice and ~30-minute continuous imaging in flies. Using dual-polarity multiplexed imaging, we uncovered brain state-dependent antagonism between neocortical somatostatin-expressing (SST+) and va... More

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