Products/Services Used | Details | Operation |
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Proteins, Expression, Isolation and Analysis | according to the manufacturer’s recommendations and grown shaking in suspension in a humidified incubator at 37°C and 8% CO2. On day 5 after transfection, AmMag Ni Magnetic Beads (GenScript, Piscataway, NJ) | Get A Quote |
Tools to monitor SARS-CoV-2 transmission and immune responses are needed. We present a neutralization ELISA to determine the levels of Ab-mediated virus neutralization and a preclinical model of focused immunization strategy. The ELISA is strongly correlated with the elaborate plaque reduction neutralization test (ρ = 0.9231, < 0.0001). The neutralization potency of convalescent sera strongly correlates to IgG titers against SARS-CoV-2 receptor-binding domain (RBD) and spike (ρ = 0.8291 and 0.8297, respectively; < 0.0001) and to a lesser extent with the IgG titers against protein N (ρ = 0.6471, < 0.0001). The preclinical vaccine NMRI mice models using RBD and full-length spike Ag as immunogens show a prof... More