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Proteins, Expression, Isolation and Analysis | … The relative quantification in gene expression was determined using the 2 −ΔΔCt … Blotted proteins were incubated with anti-Fks1 primary antibody (GenScript Biotech, Piscataway, NJ … | Get A Quote |
Echinocandin drugs have become a front-line therapy against spp. infections due to the increased incidence of infections by species with elevated azole resistance, such as . Echinocandins target the fungal-specific enzyme ß-(1,3)-glucan synthase (GS), which is located in the plasma membrane and catalyzes the biosynthesis of ß-(1,3)-glucan, the major component of the fungal cell wall. However, resistance to echinocandin drugs, which results from hotspot mutations in the catalytic subunits of GS, is an emerging problem. Little structural information on GS is currently available because, thus far, the GS enzyme complex has resisted homogenous purification, limiting our understanding of GS as a major biosyntheti... More