Patient-Specific induced Pluripotent Stem Cell-Derived Hepatocyte-Like Cells as a Model to Study Autosomal Recessive Hypercholesterolemia.(No resources)
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Proteins, Expression, Isolation and Analysis |
… The top 3 off-target regions of sgRNA 1 and 2 were determined using the CRISPR genome editing
tool from IDT. Primers were designed … Seventy µg of protein were loaded on a 4%–20%
ExpressPlus PAGE Gel (Genscript), and separated via electrophoresis. Next, the … |
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