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Structure of monkeypox virus DNA polymerase holoenzyme

Science (New York, N. Y.). 2023-01; 
Qi Peng, Yufeng Xie, Lu Kuai, Han Wang, Jianxun Qi, George F Gao, Yi Shi
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Gene Synthesis The coding sequences of MPXV F8 (GenBank: URK20494.1), A22 (GenBank: URK20570.1), and E4 (GenBank: URK20538.1) were synthesized and codon-optimized using the Bac-to-Bac expression system (Genscript). Get A Quote

摘要

The World Health Organization declared mpox (or monkeypox) a public health emergency of international concern in July 2022, and prophylactic and therapeutic measures are in urgent need. The monkeypox virus (MPXV) has its own DNA polymerase F8, together with the processive cofactors A22 and E4, constituting the polymerase holoenzyme for genome replication. Here, we determined the holoenzyme structure in complex with DNA using cryo-electron microscopy at the global resolution of ~2.8 angstroms. The holoenzyme possesses an architecture that suggests a "forward sliding clamp" processivity mechanism for viral DNA replication. MPXV polymerase has a DNA binding mode similar to that of other B-family DNA polymerases fr... More

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