| Products/Services Used | Details | Operation |
|---|---|---|
| SurePAGE??, Bis-Tris, 10x8, 4-12%, 15 wells | Protein concentrations were measured with a BCA protein quantity kit (Sangon Biotech, #C503021). Approximately 25 mg of total protein from each sample was resolved by SDS-PAGE with 4% to 12% 15-well SurePAGE Gel (GenScript, #M00654) and then transferred onto polyvinylidene difluoride membranes (Millipore, #ISEQ00010) following standard procedures | Get A Quote |
| SurePAGE??, Bis-Tris, 10x8, 4-12%, 15 wells Sample (MOPS Buffer) | Get A Quote |
The pleiotropic cytokine interferon-gamma (IFNγ) is associated with cytostatic, antiproliferation, and proapoptotic functions in cancer cells. However, resistance to IFNγ occurs in many cancer cells, and the underlying mechanism is not fully understood. To investigate potential IFNγ-resistance mechanisms, we performed IFNγ-sensitivity screens in more than 40 cancer cell lines and characterized the sensitive and resistant cell lines. By applying CRISPR screening and transcriptomic profiling in both IFNγ-sensitive and IFNγ-resistant cells, we discovered that activation of double-strand break (DSB) repair genes could result in IFNγ resistance in cancer cells. Suppression of single-strand break (SSB) repair ... More
