| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | The aspB gene from Streptomyces sp. NRRL S-1868 was codon optimized, synthesized and cloned by GenScript into a pET-28a(+) expression vector with a C-terminal hexahistidine tag./The nzeB gene from Streptomyces sp. NRRL F-5053 was synthesized and cloned by Genscript into a pET-28a(+) expression vector using the restriction sites NdeI and HindIII with an Nterminal hexahistidine tag. | Get A Quote |
The cytochrome P450 (CYP) AspB is involved in the biosynthesis of the diketopiperazine (DKP) aspergilazine A. Tryptophan-linked dimeric DKP alkaloids are a large family of natural products that are found in numerous species and exhibit broad and often potent bioactivity. The proposed mechanisms for C-N bond formation by AspB, and similar C-C bond formations by related CYPs, have invoked the use of a ferryl-intermediate as an oxidant to promote substrate dimerization. Here, the parallel application of steady-state and transient kinetic approaches reveals a very different mechanism that involves a ferric-superoxide species as a primary oxidant to initiate DKP-assembly. Single turnover kinetic isotope effects and ... More
