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A Small RNA, SaaS, Promotes Salmonella Pathogenicity by Regulating Invasion, Intracellular Growth, and Virulence Factors

Microbiol Spectr .. 2023-10; 
Lin-Lin Cai, Yun-Ting Xie , Hai-Jing Hu , Xing-Lian Xu , Hu-Hu Wang , Guang-Hong Zhou
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Proteins, Expression, Isolation and Analysis SsaV was purified using glutathione MagBeads (GenScript, Nanjing, Jiangsu, China), according to protocol. Get A Quote

摘要

Salmonella enterica serovar Enteritidis is a common foodborne pathogen that infects both humans and animals. The S. Enteritidis virulence regulation network remains largely incomplete, and knowledge regarding the specific virulence phenotype of small RNAs (sRNAs) is limited. Here, we investigated the role of a previously identified sRNA, Salmonella adhesive-associated sRNA (SaaS), in the virulence phenotype of S. Enteritidis by constructing mutant (ΔsaaS) and complemented (ΔsaaS/psaaS) strains. SaaS did not affect S. Enteritidis; it was activated in the simulated intestinal environment (SIE), regulating the expression of virulence target genes. We discovered that it directly binds ssaV mRNA. Caco-2 and RAW 26... More

关键词

SaaS; Salmonella Enteritidis; bacterial attachment; intracellular growth; sRNA; virulence.