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The Peroxidase gene OsPrx114 Activated by OsWRKY50 Enhances Drought Tolerance through ROS Scavenging in Rice

Plant Physiology and Biochemistry. 2023-11; 
Chao Zheng , Xuming Wang , Yue Xu , Shaomin Wang , Xin Jiang e , Xiuli Liub , Weijun Cui , Yueyan Wu , Chengqi Yan , Hongjia Liu , Yin Lu e, Jianping Chen a,b, and Jie Zhou
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Proteins, Expression, Isolation and Analysis The samples were separated 179 by SDS-PAGE gel (Genescript, Nanjing, China) for western blot analysis and detected 180 by immunoblotting using anti-FLAG antibody (Genscript, Nanjing, China). Get A Quote

摘要

Drought is among the most severe environmental stressors, imposing detrimental effects on plant growth and development. In this study, we have identified a class III peroxidase gene, OsPrx114, which was induced under PEG (Polyethylene glycol) and drought conditions and found to be localized in both the plasma membrane and endoplasmic reticulum. The promoter region of OsPrx114 encompasses cis-elements, including ABRE (ABA response elements), MBS (MYB binding elements), and W-box, which are likely contributors to drought tolerance. In comparison to Nipponbare (Nip) plants, the overexpression of OsPrx114 enhanced drought tolerance by reducing the accumulation of reactive oxygen species (ROS) through the upregulati... More

关键词

drought tolerance; OsPrx114; OsWRKY50; rice; ROS scavenging