Cryo-EM structures of human SID-1 transmembrane family proteins and implications for their low-pH-dependent RNA transport activity
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| Codon Optimization |
The full-length cDNAs for human SIDT1 and SIDT2 (Uniport: Q9NXL6 and Q8NBJ9, respectively) were codon-optimized and synthesized by GenScript Co., Ltd./The extraction was centrifuged at 39,000 × g (Beckman) for 60 minutes to remove insoluble components, and the supernatant was incubated with anti-Flag affinity resin (GenScript Co., Ltd.) at 4 °C for 90 minutes. |
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| Peptide Synthesis |
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