| Products/Services Used | Details | Operation |
|---|---|---|
| Codon Optimization | … codon optimization was purchased from the Genscript, for recombinant protein expression in … Primary antibodies used were rabbit anti-CD4 monoclonal antibody (Sinobiological, 10400-… | Get A Quote |
The pupal cuticle protein from Aedes aegypti (AaPC) inhibits dengue virus (DENV) infection; however, the underlying mechanism of this inhibition remains unknown. Here, we report that AaPC is an intrinsically disordered protein and interacts with domain I/II of the DENV envelope protein via residues Asp59, Asp61, Glu71, Asp73, Ser75, and Asp80. AaPC can directly bind to and cause the aggregation of DENV, which in turn blocks virus infection during the virus-cell fusion stage. AaPC may also influence viral recognition and attachment by interacting with human immune receptors DC-SIGN and CD4. These findings enhance our understanding of the role of AaPC in mitigating viral infection and suggest that AaPC is a poten... More
