Products/Services Used | Details | Operation |
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Proteins, Expression, Isolation and Analysis | … the first 100 bp of the pal gene at the 5' end, and the last 100 bp plus 200 bp downstream of the pal gene at the 3' end, was synthesized and ligated into the pUC18 vector by GenScript (… | Get A Quote |
The bacterial flagellar motor is a molecular nanomachine, the assembly and regulation of which requires many accessory proteins. Their identity, structure and function are often discovered through characterisation of mutants with impaired motility. Here, we demonstrate the functional association of the Helicobacter pylori peptidoglycan-associated lipoprotein (HpPal) with the flagellar motor by analysing the motility phenotype of the ∆pal mutant, and present the results of the preliminary X-ray crystallographic analysis of its globular C-terminal domain HpPal-C. Purified HpPal-C behaved as a dimer in solution. Crystals of HpPal-C were grown by the hanging drop vapour diffusion method using medium molecular wei... More