| Products/Services Used | Details | Operation |
|---|---|---|
| Proteins, Expression, Isolation and Analysis | … Purification was performed at room temperature using Ni column (Genscript, nanjing, China) following the manufacturer's instructions. The supernatant was loaded onto Ni column, in … | Get A Quote |
Clostridium septicum alpha toxin (CSA) plays significant roles in ruminant's braxy. Genetically engineered CSA has been shown to function as a potential vaccine candidate in the prevention of the disease caused by Clostridium septicum. In the present study, we synthesized a non-toxic recombinant, rCSA by introducing four amino acid substitutions (C86L/N296A/H301A/W342A) and 11-amino-acid deletion (residues 212 to 222). Compared to recombinant CSA, rCSA showed no cytotoxicity to MDCK cells and was not fatal to mice. Moreover, rCSA could protect immunized mice against 5 × mouse LD (100% lethal dose) of crude CSA without obvious pathological change. Most importantly, rabbits immunized with rCSA produced high ti... More
