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Pyruvate kinase is post-translationally regulated by sirtuin 2 in Aedes aegypti mosquitoes

Insect Biochem Mol Biol. 2023-10; 
Natthida Petchampai, Jun Isoe, Prashanth Balaraman, Max Oscherwitz, Brendan H Carter, Cecilia G Sánchez, Patricia Y Scaraffia
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Molecular Biology Reagents … acquired from GenScript USA Inc. (Piscataway, NJ, USA). The recombinant AaPK1 protein was … In mammals, SIRT family proteins are known to have the ability to modify the acetylation … Get A Quote

摘要

We previously demonstrated that Aedes aegypti pyruvate kinase (AaPK) plays a key role in the regulation of both carbon and nitrogen metabolism in mosquitoes. To further elucidate whether AaPK can be post-translationally regulated by Ae. aegypti sirtuin 2 (AaSirt2), an NAD-dependent deacetylase that catalyzes the removal of acetyl groups from acetylated lysine residues, we conducted a series of analysis in non-starved and starved female mosquitoes. Transcriptional and protein profiles of AaSirt2, analyzed by qPCR and western blots, indicated that the AaSirt2 is differentially modulated in response to sugar or blood feeding in mosquito tissues dissected at different times during the first gonotrophic cycle. We al... More

关键词

Enzyme regulation, Glucose and ammonia metabolism, Lysine acetylation, Post-translational modification, Protein deacetylation, Starvation