Products/Services Used | Details | Operation |
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Gene Synthesis | … The genes of selected heavy chains were synthesized with human IgG1 backbone (GenScript). Kappa and lambda chains were synthesized similarly. To express mAbs plasmids were … | Get A Quote |
The success of nucleoside-modified mRNAs in lipid nanoparticles (mRNA-LNP) as COVID-19 vaccines heralded a new era of vaccine development. For HIV-1, multivalent envelope (Env) trimer protein nanoparticles are superior immunogens compared with trimers alone for priming of broadly neutralizing antibody (bnAb) B cell lineages. The successful expression of complex multivalent nanoparticle immunogens with mRNAs has not been demonstrated. Here, we show that mRNAs can encode antigenic Env trimers on ferritin nanoparticles that initiate bnAb precursor B cell expansion and induce serum autologous tier 2 neutralizing activity in bnAb precursor V + V knock-in mice. Next-generation sequencing demonstrates acquisition of ... More