| Products/Services Used | Details | Operation |
|---|---|---|
| Custom DNA/RNA Oligos | … (+) by GenScript; the sequence contains proline substitutions at residues 986 and 987 (S-2P… in Expi293F cells (ThermoFisher, Monza, Italy), purified from the cell supernatants 6 days … | Get A Quote |
Analytical ultracentrifugation (AUC) analysis shows that the SARS-CoV-2 trimeric Spike (S) protein adopts different quaternary conformations in solution. The relative abundance of the "open" and "close" conformations is temperature-dependent, and samples with different storage temperature history have different open/close distributions. Neutralizing antibodies (NAbs) targeting the S receptor binding domain (RBD) do not alter the conformer populations; by contrast, a NAb targeting a cryptic conformational epitope skews the Spike trimer toward an open conformation. The results highlight AUC, which is typically applied for molecular mass determination of biomolecules as a powerful tool for detecting functionally r... More
