Products/Services Used | Details | Operation |
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Peptide Synthesis | … We obtained these peptides from solid phase synthesis, followed by HPLC purification to a purity judged by reverse phase chromatography of at least 90% (Genscript). To screen these … | Get A Quote |
There is an arms race between beta-lactam antibiotics development and co-evolving beta-lactamases, which provide resistance by breaking down beta-lactam rings. We have observed that certain beta-lactamases tend to aggregate, which persists throughout their evolution under the selective pressure of antibiotics on their active sites. Interestingly, we find that existing beta-lactamase active site inhibitors can act as molecular chaperones, promoting the proper folding of these resistance factors. Therefore, we have created Pept-Ins, synthetic peptides designed to exploit the structural weaknesses of beta-lactamases by causing them to misfold into intracellular inclusion bodies. This approach restores sensitivity ... More