Rapid detection of in sputum using CRISPR-Cas12b combined with cross-priming amplification in a single reaction

In this study, we successfully established a new One-Pot method, named TB One-Pot, for detecting Mtb in sputum by combining CRISPR-cas12b-mediated trans-cleavage with cross-priming amplification (CPA). Our study evaluated the diagnostic performance of TB One-Pot in clinical sputum samples for tuberculosis. The findings provide evidence for the potential of TB One-Pot as a diagnostic tool for tuberculosis.

CRISPR, Cas12b, Mycobacterium tuberculosis, cross-priming amplification, nucleic acid detection