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Mosquito E-20-Monooxygenase Gene Knockout Increases Dengue Virus Replication in Cells

Viruses. 2024-03; 
Bo Li, Di Wang, Xiaoxue Xie, Xiaoli Chen, Guorui Liang, Dan Xing, Teng Zhao, Jiahong Wu, Xinyu Zhou, Chunxiao Li
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Synthetic sgRNA and crRNA Service … The sgRNA sequences were synthesized by GenScript Biotech. … gene in Aag2 cells infected with DENV2, we used qRT-PCR to assess the relative fold change (FC) of the E20MO gene … Get A Quote

摘要

E-20-monooxygenase (E20MO) is an enzymatic product of the shade (shd) locus (cytochrome p450, E20MO). Initially discovered in Drosophila, E20MO facilitates the conversion of ecdysone (E) into 20-hydroxyecdysone (20E) and is crucial for oogenesis. Prior research has implicated 20E in growth, development, and insecticide resistance. However, little attention has been given to the association between the E20MO gene and DENV2 infection. The transcriptome of Ae. aegypti cells (Aag2 cells) infected with DENV2 revealed the presence of the E20MO gene. The subsequent quantification of E20MO gene expression levels in Aag2 cells post-DENV infection was carried out. A CRISPR/Cas9 system was utilized to create an E20MO gene... More

关键词

CRISPR/Cas9, DENV2, E-20-monooxygenase, overexpression