| Products/Services Used | Details | Operation |
|---|---|---|
| Mutagenesis Services | … Site-directed mutagenesis was performed on the expression plasmid for the wild-type AcAlgE3, serving as a template, by GenScript (Jiangsu, China). The resulting plasmid encoded … | Get A Quote |
Among the epimerases specific to alginate, some of them in Azotobacter genera convert β-d-mannuronic acid to α-l-guluronic acid but also have lyase activity to degrade alginate. The remarkable characteristics of these epimerases make it a promising enzyme for tailoring alginates to meet specific demands. Here, we determined the structure of the bifunctional mannuronan C-5 epimerase AlgE3 from Azotobacter chroococcum (AcAlgE3) in complex with several mannuronic acid oligomers as well as in apo form, which allowed us to elucidate the binding manner of each mannuronic acid oligomer, and the structural plasticity, which is dependent on calcium ions. Moreover, a comprehensive analysis of the lyase activity profile... More
