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BRD9 regulates normal human hematopoietic stem cell function and lineage differentiation

Cell Death Differ. 2024-05; 
Swati Garg, Wei Ni, Basudev Chowdhury, Ellen L Weisberg, Martin Sattler, James D Griffin
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Molecular Biology Reagents … An ORF clone for GATA1(#OHu22697) was purchased and custom cloned in pLVX-EF1α-IRES-mCherry vector using GenScript reagent services. Lentiviral transduction of primary cells … Get A Quote

摘要

Bromodomain containing protein 9 (BRD9), a member of the non-canonical BRG1/BRM-associated factor (ncBAF) chromatin remodeling complex, has been implicated as a synthetic lethal target in AML but its function in normal human hematopoiesis is unknown. In hematopoietic stem and progenitor cells (HSPC) genomic or chemical inhibition of BRD9 led to a proliferative disadvantage and loss of stem cells in vitro. Human HSPCs with reduced BRD9 protein levels produced lower numbers of immature mixed multipotent GEMM colonies in semi-solid media. In lineage-promoting culture conditions, cells with reduced BRD9 levels failed to differentiate into the megakaryocytic lineage and showed delayed differentiation into erythroid ... More

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