| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | … the first 100 bp of the targeted gene, and the last 100 bp plus 200 bp downstream of the targeted gene, were synthesized and ligated into pUC18 by GenScript (USA). The ΔpilN and … | Get A Quote |
Helicobacter pylori encodes homologues of PilM, PilN and PilO from bacteria with Type IV pili, where these proteins form a pilus alignment complex. Inactivation of pilO changes H. pylori motility in semi-solid media, suggesting a link to the chemosensory pathways or flagellar motor. Here, we showed that mutation of the pilO or pilN gene in H. pylori strain SS1 reduced the mean linear swimming speed in liquid media, implicating PilO and PilN in the function, or regulation of, the flagellar motor. We also demonstrated that the soluble variants of H. pylori PilN and PilO share common biochemical properties with their Type IV pili counterparts which suggests their adapted function in the bacterial flagellar motor m... More
