| Products/Services Used | Details | Operation |
|---|---|---|
| Codon Optimization | … For Types II and VA systems, the identified Cas protein was codon-optimized for expression in Escherichia coli via the GenScript Codon optimization tool 20 and synthesized with a … | Get A Quote |
CRISPR-Cas systems have proven effective in a variety of applications due to their ease of use and relatively high editing efficiency. Yet, any individual CRISPR-Cas system has inherent limitations, necessitating a diversity of RNA-guided nucleases to suit applications with distinct needs. We searched through metagenomic sequences to identify RNA-guided nucleases and found enzymes from diverse CRISPR-Cas types and subtypes, the most promising of which we developed into gene-editing platforms. Based on prior annotations of the metagenomic sequences, we establish the likely taxa and sampling locations where Class 2 CRISPR-Cas systems active in eukaryotes may be found. The newly discovered systems show robust capa... More
