至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

Improvement and application of vacuum-infiltration system in tomato

Horticulture research. 2024-07; 
Xinghao Yao, Ayat Taheri, Hang Liu, Yaojie Zhang, Ling Li, Jin Shao, Ke Wu, Qing Miao, Weizhi He, Xinyi Hu, Kexuan Tang
Products/Services Used Details Operation
Plasmid DNA Preparation For level-0 components, CaMV35S-P + 5U and 3U + NOS-T were PCR-amplified from the pEAQ-HT-DEST plasmid using Taq DNA polymerase (GenScript Biotech Corporation, Nanjing, China). Get A Quote

摘要

Agrobacterium-mediated transient expression system has been developed and applied to various plants as alternatives to stable transformation. However, its application in tomatoes is still limited due to low expression efficiency. In this study, we described an improved vacuum-infiltration system that can be used in both tomato fruits and leaves. Notably, this study is the first report of vacuum infiltration in attached tomato fruits. The feasibility of the improved vacuum-infiltration system in Micro-Tom tomato was confirmed by various assays, including multiple fluorescent protein expression analysis, β-glucuronidase activity analysis, and RUBY reporter visualization. Subsequently, the improved vacuum-infiltr... More

关键词

Transient expression system, Vacuum infiltration, Plant biotechnology, Scutellarin, Flavonoid, Tomato