| Products/Services Used | Details | Operation |
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| Proteins, Expression, Isolation and Analysis | Hippocampal tissue was fully lysed with RIPA lysate containing phosphatase and protease inhibitors on a freeze grinder, then centrifuged at 12000 rpm at 4℃ for 15 min, and the supernatant was transferred to a clean centrifuge tube. Protein samples were isolated using 4-20% SDS-PAGE (#M00657, SurePAGE™, GenScript) and transferred to PVDF membranes using eBlot™ L1 (#L00686C, Genscript), incubated in 5%BSA at room temperature for 1 h before incubation of primary antibody (4℃, overnight; Vinculin, #13901, cell signaling technology, 1:1000; HCRTR1, #18370, Proteintech). | Get A Quote |
| eBlot ? L1 Fast Wet Transfer System | Get A Quote |
Cognitive dysfunction is not only a common symptom of major depressive disorder, but also a more common residual symptom after antidepressant treatment and a risk factor for chronic and recurrent disease. The disruption of hypocretin regulation is known to be associated with depression, however, their exact correlation is remains to be elucidated. Hypocretin-1 levels were increased in the plasma and hypothalamus from chronic unpredictable mild stress (CUMS) model mice. Excessive hypocretin-1 conducted with hypocretin receptor 1 (HCRTR1) reduced lactate production and brain8 derived neurotrophic factor (BDNF) expression by hypoxia-inducible factor-1α (HIF9 1α), thus impairing adult hippocampal neuroplas... More
