随机肽库

随机肽库（Random Library）

是序列优化必不可少的工具。随机肽库生成的特有序列有提升多肽活性的潜力。该类肽库是利用"散弹枪"法，对选取的位点利用20种天然氨基酸进行随机自发取代而得到的。

应用领域

Overlapping Peptide Alanine Scanning Truncation Library Positional Scanning Random Library Scrambled Library T Cell Truncated Library

参考文献

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Menendez A, and Scott JK. The nature of target-unrelated peptides recovered in the screening of phage-displayed random peptide libraries with antibodies. Anal. Biochem. Jan 2005 15; 336(2): 145-57.



Irving MB, Pan O, and Scott JK. Random-peptide libraries and antigen-fragment libraries for epitope mapping and the development of vaccines and diagnostics. Curr. Opin. Chem. Biol. Jun 2001; 5(3): 314-24.



Yang M, Wu Z, and Fields S. Protein-peptide interactions analyzed with the yeast two-hybrid system. Nucleic Acids Res. Apr 1995; 11; 23(7): 1152-6.