截头肽库

截头肽库（Truncation Peptide Library）

用以确定抗原表位的最短长度，通过系统性地截取两侧末端氨基酸获得。一般与丙氨酸筛查肽库结合使用，在使用丙氨酸确定表位关键残基后，截头肽库用来进一步确定最短表位序列及其作用。

在许多案例中，截头肽库用于可鉴定筛选增强蛋白水解作用的多肽。它可以作为研究多肽药物代谢降解程度的工具。

应用领域

Overlapping Peptide Alanine Scanning Truncation Library Positional Scanning Random Library Scrambled Library T Cell Truncated Library

参考文献

Svenson J, Stensen W, Brandsdal BO, Haug BE, Monrad J, and Svendsen JS. Antimicrobial peptides with stability toward tryptic degradation. Biochemistry. Mar 2008 25; 47(12): 3777-88.



Bolger GB, Baillie GS, Li X, Lynch MJ, Herzyk P, Mohamed A, Mitchell LH, McCahill A, Hundsrucker C, Klussmann E, Adams DR, and Houslay MD. Scanning peptide array analyses identify overlapping binding sites for the signalling scaffold proteins, beta-arrestin and RACK1, in cAMP-specific phosphodiesterase PDE4D5. Biochem. J. Aug 2006; 15; 398(1): 23-36.



Ostermeier M, Nixon AE, Shim JH, and Benkovic SJ. Combinatorial protein engineering by incremental truncation. Proc. Natl. Acad. Sci. U S A. Mar 1999 30; 96(7): 3562-7.



de Figueiredo P, Roberts RL, and Nester EW. DARTs: A DNA-based in vitro polypeptide display technology. Proteomics. Oct 2004; 4(10): 3128-40.



Horswill AR, Naumann TA, and Benkovic SJ. Using incremental truncation to create libraries of hybrid enzymes. Methods Enzymol. 2004; 388: 50-60.